Using multiplex analysis, we performed a comparative study of cytokine and growth factor production by human umbilical cord tissue-derived multipotent mesenchymal stromal cells (UC-MSC) cultured under standard conditions and in the presence of human umbilical cord blood serum (UCBS). It was found that the secretion of most studied molecules, including well-known inductors of regeneration HGF, G-CSF, GM-CSF, and VEGF by UCMSC considerably increased in the presence of 5% UCBS. The use of UCBS allows not only obtaining xenogenic-free cellular and cell-free therapeutic products, but also increasing the secretion of most biologically active molecules capable of stimulating repair processes.
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 84-88, June, 2020
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Romanov, Y.A., Vtorushina, V.V., Dugina, T.N. et al. Human Umbilical Cord Tissue-Derived Multipotent Mesenchymal Stromal Cells Exhibit Maximum Secretory Activity in the Presence of Umbilical Cord Blood Serum. Bull Exp Biol Med 169, 544–548 (2020). https://doi.org/10.1007/s10517-020-04926-2
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DOI: https://doi.org/10.1007/s10517-020-04926-2